What is Biochip Array Technology?
Dr Wim Reybroek, ILVO Melle - Belgium
Randox have created the world’s first Biochip Array Technology analyser. The Evidence Investigator is a sophisticated semi-automated biochip system perfectly designed for research, clinical, forensic and veterinary applications.
The biochip is used to simultaneously analyse a panel of related tests in a single sample producing a patient profile. The surface chemistry of the biochip and the analysis platforms allow BAT to be used with a range of samples. Urine, tissue, feed, milk, seafood and eggs can all be used in conjunction with the drug residue platforms.
How does Biochip Array Technology work?
The technology is based upon ELISA principles of sandwich and competitive immunoassays.
Multiple specific ligands (antibodies or antigens) are attached at pre-defined discrete test regions on the surface of the biochip.
Analyte in a sample leads to binding of conjugate labelled with Horseradish Peroxidase (HRP). The addition of signal reagent leads to generation of a light signal.
Addition of assay reagents & sample to the biochip
Full reagent package provided. No hidden costs or extra consumables needed.
Multiple results can be obtained simultaneously from as little as 25ul of sample. Up to 54 samples can be analysed per run.
Biochips placed in custom thermoshaker
The custom thermoshaker provides the optimum-heating environment for samples. The heated lid provides faster heat-up times, bi-directional heating, increased temperature range and standardisation of assay incubation conditions.
Washing of biochips
The washing procedure is quick and easy and is performed using a wash bottle.
Biochip carrier loaded into Evidence Investigator
Signal reagent is added to each biochip before imaging.
It only takes 2 minutes for the CCD camera to image each carrier.
Discrete test sites on each biochip for individual analytes
The light signal generated from each of the discrete test regions on the biochip is simultaneously detected.
The analyser used unique image processing software to translate the light signal generated from the chemiluminescent reactions into an analyte concentration. No manual handling of data required.
Testing samples using Biochip Array Technology is simple.
Watch this video for a demonstration.