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Chloramphenicol ELISA

Catalogue No.CN1469 (Click Here to Order)

Overview

Key Advantages:

  • Excellent Cross reactivity with the major metabolite in urine.
  • Methodologies developed for a range of sample matrices
  • Rapid and simple assay format
  • Plates precoated with analyte specific antibody, reducing assay set up time
  • Exceptional Limits of Detection across the various sample matrices
  • Tissue extraction buffer helps remove the problem of emulsion which can be formed when running tissue samples.

Chloramphenicol is a broad spectrum antibiotic used to treat a wide range of infections. However, in humans it leads to Chloramphenicol induced aplastic anaemia. This has led to the use of Chloramphenicol being totally banned within the European Union since 1994 for the treatment of animals used for food production.

With the Randox Food Diagnostics Chloramphenicol (CN1469) ELISA it is possible to detect Chloramphenicol and its glucuronide (the major metabolite in urine).

Assay Limits of Detection

urine (rapid screen) 0.25ng/ml
urine (solvent extraction) 0.01ng/ml
serum (rapid screen) 0.5ng/ml
serum (solvent extraction) 0.02ng/ml
muscle 0.02ng/g
liver/kidney 1.5ng/g
feed 0.2ng/g
milk 0.1ng/ml
honey (rapid screen) 1.5ng/g
honey (solvent extraction) 0.07ppb
honey (C18 extraction) 0.1ng/g
egg 0.02ng/g
milk powder 0.08ng/ml

Assay Specificity

Chloramphenicol %Cross-Reactivity
Chloramphenicol100
Chloramphenicol glucuronide 100
Chloramphenicol Base <0.1
Chloramphenicol Palmite 0.5
Chloramphenicol Stearate 0.1
Thiamphenicol <0.5
Sulphamethazine <0.01
Monensin <0.01
Chlortetracycline <0.01
Penicillin <0.01
Tylosin <0.01
Avoparcin <0.01
Nitrofurazone <0.01
Nitrofurantoin <0.01
Furazolidone <0.01