Total Antioxidant Status
For the quantitative in vitro determination of Total Antioxidant Status in serum, plasma, wine, beer and fruit juice. This product is suitable for both manual and automated use.
The Randox Food Diagnostics Total Antioxidant Status kit can also be used to determine the phenolic content of beverages such as wine, beer and fruit juice. Antioxidants present in red wine, tea and other beverages have been shown to give a cardio-protective effect. The compounds in beverages, which contribute to this effect, are Phenolics, of which Polymeric Phenols are the largest subgroup. Phenolics also contribute towards the taste, colour, odour and preservative of the beverage.
ABTS® (2, 2’-Azino-di-[3-ethylbenzthiazoline sulphonate]) is incubated with a peroxidase (metmyoglobin) and H2O2 to produce the radical cation ABTS®*+. This has a relatively stable blue-green colour, which is measured at 600 nm. Antioxidants in the added sample cause suppression of this colour production to a degree which is proportional to their concentration.
|Lyophilised kit for enhanced stability of two years from date of manufacture||Cost savings with maximised number of tests per kit||Advanced chemistry but simple operator input ensures minimal test errors|
- MSDS sheet
- Kit insert
- Calculation Spreadsheet
- Instrument specific protocols on request for automated analysers - Request »
Testing for Antioxidants in Wine, Tea and Food
The TAS kit from Randox Food Diagnostics measures the antioxidant activity in beverages and provides a cost-effective method for rapid analysis of samples. This kit provides a useful tool for beverage companies to evaluate the phenolic content of their products, as phenolics appear to account for almost all the antioxidant activity in many beverages. The TAS Kit comprising unique patented technology and is the only commercially available test in complete kit format which measures the total antioxidant status. The assay is suitable for manual and automated use with results available in three minutes.
Our TAS kit utilises the following highly specific enzymatic reaction:
ABTS® is incubated with a peroxidase (metmyoglobin) and H2O2 to produce the radical cation ABTS®*+. This has a relatively stable blue-green colour, which is measured at 600 nm. Antioxidants in the added sample cause suppression of this colour production to a degree which is proportional to their concentration.