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Nortestosterone ELISA

Catalogue No. NT2105 (Click Here to Order)

Overview

Key Advantages:

  • Also detects 17β Trenbolone
  • Rapid and simple assay format
  • Plates precoated with analyte specific antibody, reducing assay set up time.
  • Exceptional Limits of Detection
  • Accessory Immunoaffinity Columns available for sample clean up allowing higher signal to noise ratios and cleaner samples suitable for ELISA, HPLC or GC-MS

19-Nortestosterone is an anabolic steroid used as a growth promoting agent in the production of meat.  19-Nortestosterone is frequently abused illegally in animal breeding.

Whilst steroids result in a higher meat/fat ratio, there is the risk that residues will be left in meat products and be harmful to the consumer.  The use of nortestosterone and its derivatives is banned by the EC for use in livestock production .  

Assay Limits of Detection

Serum 1.0ng/ml
Tissue 2ng/ml  
Urine (rapid screen) 3ng/ml 
Urine (C18 method) 0.8ng/ml

Assay Specificity

Compound % Cross-Reactivity
19-Nortestosterone (17β) 100
Trenbolone (17β) 85
Trenbolone Acetate 25
Norethisterone 7.4
19-Nor-4-Androstene,-3,17-Dione 186
19-Nortestosterone (17α) 50
19-Nortestosterone (17β) Sulphate 40
Boldenone < 0.2
Progesterone 1.3
Dihydrotestosterone 0.2
Testosterone (17β) 0.8
Testosterone (17α) 0.2
Pregnenolone < 0.1
Androstenedione 1.1
Cortisol 0.02
6αMethyl17αHydroxyprogesterone 2.5
Androstenediol < 0.1
4-Androsten-11βOL-3,17-Dione < 0.1
17 βestradiol 0.03
5βPregnane - 3α20α-Diol < 0.1
Hydroxyprogesterone 0.2
Estriol < 0.1
Estrone < 0.1

Used in conjunction with Trenbolone/19-Nortestosterone Immunoaffinity Column