Home Products ELISA Residue Kits Anti-microbial Sulphamethoxazole

Sulphamethoxazole ELISA

Cat. No: SZ3471 (Click Here to Order)

Overview

Key Advantages:

  • User friendly kit format
  • Simple and rapid sample preparation
  • Exceptional Limits of detection
  • Plates pre-coated with analyte specific antibody, reducing assay set up time
  • Fast, reliable, analytical method

The sulphonamides (ie. sulphamethoxazole) are competitive inhibitors of the enzyme dihydropteroate synthetase in the folic acid metabolism cycle, which inhibit bacterial growth by preventing p-aminobenzoic acid incorporation into folic acid.

The sulphonamides have a bacteriostatic effect on a broad range of gram-positive and gram-negative organisms. Sulphamethoxazole is metabolised to N4-acetyl sulphamethoxazole, the ratio of which varies with species, tissue, age and route of administration.

Sulphamethoxazole is extensively used in veterinary medicine for the prophylaxis and treatment of infectious diseases in fish and shrimp and as feed additives to promote growth in food producing animals. Honey has been found to be adulterated with antibiotics, which has led to import bans.

Assay Limits of Detection

Sulphamethoxazole4.77 ( Honey (Diluted 1/20) ng/g )

Assay Specificity

Compound %Cross-Reactivity
Sulphamethoxazole100
Dapsone <0.547
Sulphacetamide <0.547
Sulphaguanidine <0.547
Sulphamonomethoxine <0.572
Sulphatroxazole <0.572
Sulphamoxole <0.572
Sulphanilamide <0.572
Sulphanitran <0.572
Sulphaphenazole <0.576
Sulphaquinoxaline <0.576